How to make Dell Latitude E6410 dual boot

My E6410 is pre-installed with Windows 7 professional but my software required windows XP to run. The following steps were necessary for making it dual boot.

1. Resize original Windows 7 partition to 40G (a C: partition) and create a new ntfs primary partition (a D: partition) using Gparted

2. Install windows XP professional. You can get an old winXP pro CD you have, borrow it or download it.

3. With Windows XP installed on D: drive, download easyBCD AND dotnetfx 2.0 Install dotnetfx 2.0 first then easyBCD.

4. Run easy and Add New Entry, and select "Windows 7" as type and C:\ as drive. Finally click Add Entry.

5. In Bootloader setup choose "Install widows 7 bootloader on MBR" and click "write MBR".

6. Exit easyBCD and reboot in to windows 7.

7. copy the make and model of hardware (network card, video, wirteless, etc using device manager). Then download at least the wireless and network. As of Nov 26, 2010, the network card is Intel 82577LM Gigabit Network

Preparation of 1ppm aspartam solution for MTQ injection

1 sachet of Equal contains 36mg of aspartam.

1 sacket in 500mL of water gives 36mg/600mL concentration = 0.06 mg/mL = 60ppm in water.

Add 10uL of this solution to 590 uL of water gives 1ppm solution of aspartam.

How to update ncbi database on MASCOT

Download ncbi nr database from the following link. Download the nr.gz file. Once done, take the following steps.
1. Extract the nr.gz. Copy the file to D:\NETPUB\mascot\sequence\ncbinr\incoming. Rename the extracted file to ncbinr_YYYYMMDD.fasta, where YYYYMMDD is the current date.
2. On database maintenance page, select ncbinr and set it to "inactive". Click OK.
3. Move the files in D:\NETPUB\mascot\sequence\ncbinr\current to D:\NETPUB\mascot\sequence\ncbinr\old. Then, move the file in 1 to D:\NETPUB\mascot\sequence\ncbinr\current.
4. Finally, wait until the file is indexed, go back to database maintenance page and set the status of ncbinr database to "active".

Useful Opensource software packages; they work very well and are free

Useful Opensource program; it is free and legal

Openoffice.org is a capable and MS Office (largely) compatible office suite
http://www.openoffice.org/

pdfcreator allows user to create pdf file directly from any software.
http://sourceforge.net/projects/pdfcreator/

wincdemu for emulating a CD. Useful for exploring CD image file.
http://sourceforge.net/projects/wincdemu/

TrueCrypt for encrypting your file.
http://www.truecrypt.org/

gnupg encrypt your email and keep it secure
http://www.gnupg.org/
http://www.gpg4win.org/

Infrarecorder, a CD/DVD utility, is better than commercial.
http://infrarecorder.org/


Freedownloadmanager (FDM) for fast download of large file. The same as flashget, but free
http://www.freedownloadmanager.org/

Find more useful opensource software for your application at http://www.osalt.com

terminal usage fee for Manila is 750 peso

17.25 USD Nov 20

Workflow for Quantitation on MTQ

Things to ptimize transfer, then ionization and finally calibration.

1. Prepare 1 ppm solution of analyte standard in 50:50 Water:MeOH or ACN.
2. Infuse and optimize parameter to maximize signals
CC RF, Q lowmass, Transfer time (add EIC of compound of interest). (flow rate 180-240 uL/hr)
3. With a Tee, connect HPLC flowing with mobile phase at the desired flow rate. Optimize nebulizer gas and dry gas.
4. Add calibration segment and adjust the run time.
5. Remove the Tee and infuse calibrant and calibrate the instrument.
6. Save method as.

Note. Changing transfer time to maximize signal may shorten the mass range. The calibration mode may have to be adjusted. (for Caffeine, we used NaFormate and had to use TOF calibration version 2 (with Enhanced Quadratic as calibration mode) because the mass range only extends from 158 to 400 and only a few calibration points are visible.

Why my signals surge when I increase the nebulizer gas?

We infused 1ppm caffeine to MTQ with standard spray condition for infusion. The signal disappears slowly when initially sprayed to the system. Nothing we do will improve the signal, until we increase nebulizer gas pressure from 0.5 to 1.0 then 2, and 3 bars. Each time signal surged and then drop. Later we found that it was because the syringe pump was not working. The surge of signal was due to the nebulizer gas drawing the same from the spray needle.

How to deal with flies--borrowed from post below

ลูกเกษตร~
Posted : 2009-06-24 22:12:39 IP : (115.67.60.228)

กับดักแมลงวัน มีวิธีการทำที่ง่ายและประหยัด ดังต่อไปนี้
วัสดุและอุปกรณ์
1. ขวดน้ำดื่มพลาสติกแบบใส
2. กรรไกร
3. คัตเตอร์
4. เทปกาวสีดำที่ใช้พันสายไฟ

วิธีทำ
1. ใช้คัตเตอร์ตัดขวดน้ำพลาสติกออกเป็น 2 ส่วน ตัดให้ ส่วนบนวัดตั้งแต่ปากขวดลงมา สูงประมาณ 14 ซม. นำส่วนบนด้านคอขวดคว่ำสวมเข้าไปในขวดน้ำส่วนล่าง นำเทปกาวสีดำปิดให้ขวด
2. ส่วนติดสนิท และติดเทปกาวให้ได้ขอบสูงประ- มาณ 2-3 นิ้ว
3. ตัดช่องเจาะรูบริเวณที่ติดเทปกาวสีดำ จะเจาะเป็นรู กลม รูสี่เหลี่ยมก็ได้ โดยเจาะให้กว้างพอที่แมลงวันบินเข้าไปได้สะดวก
4. หาภาชนะใส่เหยื่อเพื่อล่อแมลงวัน ซึ่งอาจใช้ถ้วยเล็ก ๆ แล้วนำเปลือกกุ้ง เศษเนื้อปลาที่มีกลิ่นเหม็นใส่ลงไป เพราะกลิ่นของ เศษอาหารจะเป็นตัวล่อแมลงวันให้บินเข้าไปในกับดัก เครื่องดักจับแมลงวัน นี้ติดเทปกาวสีดำบังแสง เมื่อแมลงวันบินเข้าไปกินเศษอาหาร โดยตาม ธรรมชาติของแมลงวันจะบินขึ้นสู่ที่สูงในแนวตั้งเหมือนเฮลิคอปเตอร์ ขวด พลาสติกสีใส เมื่อแมลงวันแหงนมองดูก็คิดว่าเป็นท้องฟ้า จึงบินขึ้นไปติดกับ ดักและขังตัวอยู่ข้างบน ซึ่งแมลงวันไม่สามารถบินหลุดผ่านช่องคอขวดลงมา ได้ เพราะมันจะบินขึ้นข้างบนอย่างเดียว กับดักแมลงวันนี้ เมื่อมีแมลงวัน บินเข้าไปจนเต็ม จึงนำไปกำจัดโดยการนำไปผึ่งแดดสักพักแมลงวันก็จะตาย ไปในที่สุด การทำกับดักแมลงวันสามารถทำได้อย่างง่าย ๆ โดยใช้วัสดุที่หา ได้ง่ายและประหยัด อีกทั้งได้ผลในการกำจัดแมลงวัน

If your DVD-RW does not burn, may be this blog can help.

DVD-RW at a user site refused to write. After searching for solution, the comment below fixed the problem.

Right-Click on the drive w/ the problem
Properties
Recording Tab
and then check the "Enable CD Recording On This Drive" box.
and that fixed it.i'm running XP home w/ SP1. try it out!

Mine was "Enable", so I toggled it on and off. Then it seems to work.

Thanks!
----------------quoted--- message
crickton
Guest
.
.
.
Re:D:/ not accessible - Incorrect Function
« Reply #11 on: July 08, 2004, 02:57:11 pm »
I just started getting this error (acouple of weeks ago) and I wasn't sure what was going on. I don't have Roxio installed and have been using Nero for forever. I wasn't sure why it started messing up. I googled a bit and came to first the Microsoft site (which didn't work/wasn't applicable to me) and then this thread (which was useless and didn't apply to me). I dinked around on my comp and eventually fixed my problem. the rest of you may wanna try this out. not sure why it fixed it, or if it'll fix yours, but here goes:

Right-Click on the drive w/ the problem
Properties
Recording Tab
and then check the "Enable CD Recording On This Drive" box.

and that fixed it.
i'm running XP home w/ SP1. try it out!

Parsing rule for Spirulina amino acid database at biotec

1. Open the file to be converted with windows wordpad.exe and save the file as "Text-document MS Dos format" with extension *.faa (in this case Spirulina_KMITT.faa".

2. Create a folder
C:\inethub\mascot\sequence\Spirulina_KMITT\current

2. Copy the Spirulina_KMITT.faa to the folder above.

3. Open database maintenance

4. Click New Definition

5. Type in Name "Spirulina_KMITT".
Notice "Select" will change to the same name.
Select "Inactive"
Select "AA"
check Mem map
Taxonomy "none"

6. Path = C:/inethub/mascot/sequence/Spirulina_KMITT/current/Spirulina_KMITT.faa

7. Rule to parse accesstion string from Fasta file = rule 33

8. Rule to parse description string from Fasta file = rule 34

9. Rule to parse accession string from local reference file: --no local reference file--
Host and path are empty

10. Click "Test this definition" to test the new rule. We should obtain two column of accession number and description

11. Select "Active"

12. Select APPLY, done.

More details on the syntax of the file:

Spirulina_KMITT

C:\Inetpub\mascot\sequence\Spirulina_KMITT\current

>AP01000002/Possible HNH endonuclease: reverse transcriptase
MVRLIETMYPKKANRVQASLVRYADDFVVISPSLDIIEPCKNAIFEWLKPVGLEIKPEKTRVCHTLNPIQYEGRTEEAGFDLLGFNIRQYPVGKYKSGKTGGTASRLIGHKTHIKPSKKAVQAHTEVIKGVIKQHKTAPQSALISRLNPIIRGWENYYSGVVSSETFSKLDDIIWQMLRAWKVSRCGKANIEKLRNYLRPGTVILSNGKERHETWLFRTKDGLQLWKHNWTPIVRHTLIKPEATPYDGNWTYWSNRKGQAIGTPNRVAKLLKKQKGKCTWCGQYLPPYDLVEVDHIVP

>AP01020001/conserved hypothetical protein
MEIIKLKIRADGEGKVILQVPQDLANQELEIAVIYQSASPPSATQTPDELGWPPGFFEQTAGCLADEPLVRYDPGEYQVREEIE
.
.
.

Parsing Rules:

Rule 33: ">\([A-Z]*[0-9]*\)"
Rule 34: "/\(.*\)"

Opiate Mix

Codeine
EPI Water Solubility mg/L: 1.215e4; Polar Surface Area: 30.93 A2
http://www.chemspider.com/Chemical-Structure.4447447.html

Methadone
EPI Water Solubility: 48.81; Polar Surface Area: 20.31 A2
http://www.chemspider.com/Chemical-Structure.3953.html

Hydrocodone
EPI Water Solubility: 1788; Polar Surface Area: 38.77 A2
http://www.chemspider.com/Chemical-Structure.4447623.html

Oxycodone
EPI Water Solubility: 2.764e4; Polar Surface Area: 48 A2
http://www.chemspider.com/Chemical-Structure.4447649.html

Meperidine(Pethidine)
EPI Water Solubility: 1185; Polar Surface Area: 29.54 A2
http://www.chemspider.com/Chemical-Structure.3918.html

Barbiturate mix 5

Amobarbital
EPI WS = 544; PSA 57.69
http://www.chemspider.com/Chemical-Structure.2079.html

Butalbital
EPI WS 829.7; PSA 57.67
http://www.chemspider.com/Chemical-Structure.2387.html

Pentobarbital
EPI WS 512; PSA 57.69
http://www.chemspider.com/Chemical-Structure.4575.html

Phenobarbital
EPI WS 1644; PSA 57.67
http://www.chemspider.com/Chemical-Structure.4599.html

Secobarbital
EPI WS 570; PSA 57.69
http://www.chemspider.com/Chemical-Structure.5005.html

Benzodiazepine mix

Alprazolam
EPI Water Solubility (mg/L) = 13.1; Polar Surface Area (A2) = 40.3
http://www.chemspider.com/Chemical-Structure.2034.html

Clonazepam
EPI WS = 35.38; PSA = 78.49
http://www.chemspider.com/Chemical-Structure.2700.html

Diazepam
EPI WS = 58.78; PSA = 32.67
http://www.chemspider.com/Chemical-Structure.2908.html

Flunitrazepam
EPI WS = 72.77; PSA = 78.49
http://www.chemspider.com/Chemical-Structure.3263.html

Nordiazepam
EPI WS = 8.666 ; PSA = 32.67
http://www.chemspider.com/Chemical-Structure.2890.html

Nitrazepam
EPI WS = 76.91 ; PSA = 78.49
http://www.chemspider.com/Chemical-Structure.4350.html

Oxazepam
EPI WS = 20.71; PSA 41.9A
http://www.chemspider.com/Chemical-Structure.4455.html

Temazepam
EPI WS = 163.9; PSA 41.9A
http://www.chemspider.com/Chemical-Structure.5198.html

Triazolam
EPI WS = 4.53; PSA 43.07A
http://www.chemspider.com/Chemical-Structure.5355.html

Prazepam
EPI WS = 5.722; PSA 32.07A
http://www.chemspider.com/Chemical-Structure.4721.html

Lorazepam
EPI WS = 3.666; PSA 32.12A
http://www.chemspider.com/Chemical-Structure.3821.html

Natural Abundance of Stable Isotope

http://www.sisweb.com/referenc/source/exactmaa.htm

Name         Symbol      Mass     Abund.    Symbol     Mass      Abund.   Symbol      Mass      Abund.
----------   -------  ----------  ------    -------  ----------  ------   -------  ----------   ------
Aluminum     Al(27)    26.981541  100.00                                                            
Antimony     Sb(121)  120.903824   57.30    Sb(123)  122.904222   42.70                              
Argon        Ar(36)    35.967546    0.34    Ar(38)    37.962732   0.063    Ar(40)    39.962383   99.60
Arsenic      As(75)    74.921596  100.00                                                            
Barium       Ba(130)  129.906277    0.11    Ba(132)  131.905042    0.10    Ba(134)  133.904490    2.42
            Ba(135)  134.905668    6.59    Ba(136)  135.904556    7.85    Ba(137)  136.905816   11.23
            Ba(138)  137.905236   71.70                                                            

Beryllium    Be(9)      9.012183  100.00                                                            
Bismuth      Bi(209)  208.980388  100.00                                                            
Boron        B(10)     10.012938   19.80    B(11)     11.009305   80.20                              
Bromine      Br(79)    78.918336   50.69    Br(81)    80.916290   49.31                              
Cadmium      Cd(106)  105.906461  1.25  Cd(108)  107.904186  0.89   Cd(110)  109.903007   12.49    Cd(111)  110.904182   12.80
            Cd(112)  111.902761   24.13    Cd(113)  112.904401   12.22    Cd(114)  113.903361   28.73
            Cd(116)  115.904758    7.49

Calcium      Ca(40)    39.962591   96.95    Ca(42)    41.958622    0.65    Ca(43)    42.958770    0.14
            Ca(44)    43.955485   2.086    Ca(46)    45.953689   0.004    Ca(48)    47.952532    0.19
Carbon       C(12)     12.000000   98.90    C(13)     13.003355    1.10                              
Cerium       Ce(136)  135.907140    0.19    Ce(138)  137.905996    0.25    Ce(140)  139.905442   88.48
            Ce(142)  141.909249   11.08                                                            
Cesium       Cs(133)  132.905433  100.00                                                            
Chlorine     Cl(35)    34.968853   75.77    Cl(37)    36.965903   24.23                              

Chromium     Cr(50)    49.946046    4.35    Cr(52)    51.940510   83.79    Cr(53)    52.940651    9.50
            Cr(54)    53.938882    2.36                                                            
Cobalt       Co(59)    58.933198  100.00                                                            
Copper       Cu(63)    62.929599   69.17    Cu(65)    64.927792   30.83                              
Dysprosium   Dy(156)  155.924287   0.060    Dy(158)  157.924412    0.10    Dy(160)  159.925203    2.34
            Dy(161)  160.926939   18.90    Dy(162)  161.926805   25.50    Dy(163)  162.928737   24.90
            Dy(164)  163.929183   28.20                                                            
Erbium       Er(162)  161.928787    0.14    Er(164)  163.929211    1.61    Er(166)  165.930305   33.60
            Er(167)  166.932061   22.95    Er(168)  167.932383   26.80    Er(170)  169.935476   14.90


Europium     Eu(151)  150.919860   47.80    Eu(153)  152.921243   52.20                              
Fluorine     F(19)     18.998403  100.00                                                            
Gallium      Ga(69)    68.925581   60.10    Ga(71)    70.924701   39.90                              
Gadolinium   Gd(152)  151.919803   0.200    Gd(154)  153.920876    2.18    Gd(155)  154.822629   14.80
            Gd(156)  155.922130   20.47    Gd(157)  156.923967   15.65    Gd(158)  157.924111   24.84
            Gd(160)  159.927061   21.86                                                            
Germanium    Ge(70)    69.924250   20.50    Ge(72)    71.922080   27.40    Ge(73)    72.923464    7.80
            Ge(74)    73.921179   36.50    Ge(76)    75.921403    7.80                              

Gold         Au(197)  196.966560  100.00                                                            
Hafnium      Hf(174)  173.940065    0.16    Hf(176)  175.941420    5.20    Hf(177)  176.943233   18.60
            Hf(178)  177.943710   27.10    Hf(179)  178.945827   13.74    Hf(180)  179.946561   35.20
Helium       He(3)      3.016029   .0001    He(4)      4.002603  100.00                              
Holmium      Ho(165)  164.930332  100.00                                                            
Hydrogen     H(1)       1.007825   99.99    H(2)       2.014102   0.015  

Indium       In(113)  112.904056    4.30    In(115)  114.903875   95.70                              
Iodine       I(127)   126.904477  100.00                                                            
Iridium      Ir(191)  190.960603   37.30    Ir(193)  192.962942   62.70                              
Iron         Fe(54)    53.939612    5.80    Fe(56)    55.934939   91.72    Fe(57)    56.935396    2.20
            Fe(58)    57.933278    0.28                                                            
Krypton      Kr(78)    77.920397    0.35    Kr(80)    79.916375    2.25    Kr(82)    81.913483   11.60
            Kr(83)    82.914134   11.50    Kr(84)    83.911506   57.00    Kr(86)    85.910614   17.30

Lanthanum    La(138)  137.907114    0.09    La(139)  138.906355   99.91                              
Lead         Pb(204)  203.973037    1.40    Pb(206)  205.974455   24.10    Pb(207)  206.975885   22.10
            Pb(208)  207.976641   52.40                                                            
Lithium      Li(6)      6.015123    7.42    Li(7)      7.016005   92.58                              
Lutetium     Lu(175)  174.940785   97.40    Lu(176)  175.942694    2.60                              
Magnesium    Mg(24)    23.985045   78.90    Mg(25)    24.985839   10.00    Mg(26)    25.982595   11.10

Manganese    Mn(55)    54.938046  100.00                                                            
Mercury      Hg(196)  195.965812    0.15    Hg(198)  197.966760   10.10    Hg(199)  198.968269   17.00
            Hg(200)  199.968316   23.10    Hg(201)  200.970293   13.20    Hg(202)  201.970632   29.65
            Hg(204)  203.973481    6.80                                                            
Molybdenum   Mo(92)    91.906809   14.84    Mo(94)    93.905086    9.25    Mo(95)    94.905838   15.92
            Mo(96)    95.904676   16.68    Mo(97)    96.906018    9.55    Mo(98)    97.905405   24.13
            Mo(100)   99.907473    9.63                                                            
Neodymium    Nd(142)  141.907731   27.13    Nd(143)  142.909823   12.18    Nd(144)  143.910096   23.80
            Nd(145)  144.912582    8.30    Nd(146)  145.913126   17.19    Nd(148)  147.916901    5.76
            Nd(150)  149.920900    5.64                                                            
Neon         Ne(20)    19.992439   90.60    Ne(21)    20.993845    0.26    Ne(22)    21.991384    9.20

Nickel       Ni(58)    57.935347   68.27    Ni(60)    59.930789   26.10    Ni(61)    60.931059    1.13
            Ni(62)    61.928346    3.59    Ni(64)    63.927968    0.91                              
Niobium      Nb(93)    92.906378  100.00                                                            
Nitrogen     N(14)     14.003074   99.63    N(15)     15.000109    0.37                              
Osmium       Os(184)  183.952514    0.02    Os(186)  185.953852    1.58    Os(187)  186.955762    1.60
            Os(188)  187.955850   13.30    Os(189)  188.958156   16.10    Os(190)  189.958455   26.40
            Os(192)  191.961487   41.00                                                            
Oxygen       O(16)     15.994915   99.76    O(17)     16.999131   0.038    O(18)     17.999159    0.20

Palladium    Pd(102)  101.905609    1.02    Pd(104)  103.904026   11.14    Pd(105)  104.905075   22.33
            Pd(106)  105.903475    27.33   Pd(108)  107.903894   26.46    Pd(110)  109.905169   11.72
Phosphorus   P(31)     30.973763  100.00                                                            
Platinum     Pt(190)  189.959937   0.010    Pt(192)  191.961049    0.79    Pt(194)  193.962679   32.90
            Pt(195)  194.964785   33.80    Pt(196)  195.964947   25.30    Pt(198)  197.967879    7.20
Potassium    K(39)     38.963708   93.20    K(40)     39.963999   0.012    K(41)     40.961825    6.73
Praseodymium Pr(141)  140.907657  100.00                                                            

Rhenium      Re(185)  184.952977   37.40    Re(187)  186.955765   62.60                              
Rhodium      Rh(103)  102.905503  100.00                                                            
Rubidium     Rb(85)    84.911800   72.17    Rb(87)    86.909184   27.84                              
Ruthenium    Ru(96)    95.907596    5.52    Ru(98)    97.905287    1.88    Ru(99)    98.905937   12.70
            Ru(100)   99.904218   12.60    Ru(101)  100.905581   17.00    Ru(102)  101.904348   31.60
            Ru(104)  103.905422   18.70                                                            
Samarium     Sm(144)  143.912009    3.10    Sm(147)  146.914907   15.00    Sm(148)  147.914832   11.30
            Sm(149)  148.917193   13.80    Sm(150)  149.917285    7.40    Sm(152)  151.919741   26.70
            Sm(154)  153.922218   22.70                                                            

Scandium     Sc(45)    44.955914  100.00                                                            
Selenium     Se(74)    73.922477    0.90    Se(76)    75.919207    9.00    Se(77)    76.919908    7.60
            Se(78)    77.917304   23.50    Se(80)    79.916521   49.60    Se(82)    81.916709    9.40
Silicon      Si(28)    27.976928   92.23    Si(29)    28.976496    4.67    Si(30)    29.973772    3.10
Silver       Ag(107)  106.905095   51.84    Ag(109)  108.904754   48.16                              
Sodium       Na(23)    22.989770  100.00                                                            

Strontium    Sr(84)    83.913428    0.56    Sr(86)    85.909273    9.86    Sr(87)    86.908902    7.00
            Sr(88)    87.905625   82.58                                                            
Sulfur       S(32)     31.972072   95.02    S(33)     32.971459    0.75    S(34)     33.967868    4.21
            S(36)     35.967079   0.020                                                            
Tantalum     Ta(180)  179.947489   0.012    Ta(181)  180.948014   99.99                              
Tellurium    Te(120)  119.904021 0.096  Te(122)  121.903055    2.60    Te(123)  122.904278    0.91
            Te(124)  123.902825    4.82 Te(125)  124.904435    7.14    Te(126)  125.903310 18.95
            Te(128)  127.904464   31.69 Te(130)  129.906229   33.80                                                            
Terbium      Tb(159)  158.925350  100.00                                                            

Thallium     Tl(203)  202.972336   29.52    Tl(205)  204.974410   70.48                              
Thorium      Th(232)  232.038054  100.00                                                            
Thulium      Tm(169)  168.934225  100.00                                                            
Tin          Sn(112)  111.904826  0.97      Sn(114)  113.902784  0.65  Sn(115)  114.903348 0.36
            Sn(116)  115.901744   14.70    Sn(117)  116.902954    7.70    Sn(118)  117.901607   24.30
            Sn(119)  118.903310    8.60    Sn(120)  119.902199   32.40    Sn(122)  121.903440    4.60
            Sn(124)  123.905271    5.60                                                            
Titanium     Ti(46)    45.952633    8.00    Ti(47)    46.951765    7.30    Ti(48)    47.947947   73.80
            Ti(49)    48.947871    5.50    Ti(50)    49.944786    5.40                              

Tungsten     W(180)   179.946727    0.13    W(182)   181.948225   26.30    W(183)   182.950245   14.30
            W(184)   183.950953   30.67    W(186)   185.954377   28.60                              
Uranium      U(234)   234.040947   0.006    U(235)   235.043925    0.72    U(238)   238.050786   99.27
Vanadium     V(50)     49.947161    0.25    V(51)     50.943963   99.75                              
Xenon        Xe(124)  123.905894    0.10    X(126)   125.904281   0.09
            Xe(128)  127.903531    1.91    Xe(129)  128.904780   26.40    Xe(130)  129.903510    4.10
            Xe(131)  130.905076   21.20    Xe(132)  131.904148   26.90    Xe(134)  133.905395   10.40
            Xe(136)  135.907219    8.90                                                            
Ytterbium    Yb(168)  167.933908    0.13    Yb(170)  169.934774    3.05    Yb(171)  170.936338   14.30
            Yb(172)  171.936393   21.90    Yb(173)  172.938222   16.12    Yb(174)  173.938873   31.80
            Yb(176)  175.942576   12.70                                                            

Yttrium      Y(89)     88.905856  100.00                                                            
Zinc         Zn(64)    63.929145   48.60    Zn(66)    65.926035   27.90    Zn(67)    66.927129    4.10
            Zn(68)    67.924846   18.80    Zn(70)    69.925325    0.60                              
Zirconium    Zr(90)    89.904708   51.45    Zr(91)    90.905644   11.27    Zr(92)    91.905039   17.17
            Zr(94)    93.906319   17.33    Zr(96)    95.908272    2.78                              

Unknown error and injection stops when trying to start a run in HyStar

Unknown error and injection stops when trying to start a run in HyStar

1. Is there a sample vial in the autosampler? If not, place the sample vial. If yes, go to 2.
2. Is there a transport solvent for your uLpickup? No, place transport solvent., No, I am not using uLpickup. Go to 3.
3. RMB on Acquisition menu of HyStar and select "Commands"
4. At commands lit, LMB on +System to expand the System menu.
5. LMB on AbortBatch followed by LMB on "Execute"
6. LMB on AbortSample followed by LMB on "Execute"
7. At HyStar Acquistion page, reload sample.

Concentration of proteins for in-solution triptic digest

Typical concentration for in-solution of protein is between 1 to 2 picomole. We supply 5uL of 0.4 ug/uL for digestion. This should be sufficient.

Recipe for Sodium salts used to calibrate TOF

For Sodium formate:
10ml Water

10ml Isopropanol

40ul Formic Acid

200ul NaOH (1 molar)

All solvents must be of highest quality.

For sodium acetate:

10ml Water

10ml Isopropanol

11ul Acetic Acid

200ul NaOH (1 molar)

Preparation of a 10 mM sodium TFA calibrant solution:
12.5 mL H2O
12.5 mL isopropyl alcohol (ACN can also be used)
50 µL TFA acid (conc.)
250 µL 1 M NaOH

* I find NaOH is not always available. NaHCO3 (baking soda) 

21mg NaHCO3=250uL 1M NaOH

17mg NaHCO3=200uL 1M NaOH