Solvent Preparation for ToxTyper

Preparation of Mobile phase:

Mobile Phase A (for 100mL):

1. Water 97 mL
2. ACN 1 mL
3. 100 mM ammonium formate 2 mL
4. Conc Formic acid 0.1 mL

Mobile Phase B (for 100mL):

1. ACN 98 mL
2. 100 mM ammonium formate 2 mL
3. Conc Formic acid 0.1 mL

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Preparing 100mL of 100 mM ammomium formate

1. Add 50 mL water to volumetric flask
2. Add 381 uL of conc formic acid
3. Add 747 uL of 25% ammonia
4. Add water to make 100 mL

*** Rear Seal Wash solvent is 10 % MeOH

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Calculation for preparing ammonium formate from ammonia and formic acid

1. formic acid density = 1.22 g/l

2. concentration in % = 98-100%

3. Molecular weigth = 46

Molar conc = 10*d*x/M = 10*1.22*99%/46 = 26.25 M

1. ammonia density = 0.91 g/l

2. concentration in % = 25%

3. Molecular weigth = 17

Molar conc = 10*d*x/M = 10*0.91*25%/17 = 13.38 M

For 100mL 100mM Ammonium formate (ratio = 1:1), use formic acid = 0.01 mol => 0.01 mol/26.25M = 0.381 mL; use ammonia = 0.01 mol => 0.01 mol/13.38M = 0.747 mL