Tuesday, April 7, 2015

How to change and purge pumps

How to change solvent:
1. Start Chromeleon by double-clicking the icon below on the desktop

2. Right-click anywhere on Chromeleon to de-select "Monitor Only" and access HPLC functions. There should be no check-mark in front of "Monitor Only".






3. Set Eluent % of the pump to purge to 100%. For example, if pump B is to be purged, set B to 100% and press <return> key. 


4. Then click Purge On


5. The Purge On button is green when purge is in progress. It will run at 3 mL/min for 5 min.



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