How to change and purge pumps

How to change solvent:

1. Start Chromeleon by double-clicking the icon below on the desktop

2. Right-click anywhere on Chromeleon to de-select "Monitor Only" and access HPLC functions. There should be no check-mark in front of "Monitor Only".

3. Set Eluent % of the pump to purge to 100%. For example, if pump B is to be purged, set B to 100% and press <return> key. 

4. Then click Purge On

5. The Purge On button is green when purge is in progress. It will run at 3 mL/min for 5 min.

How to submit sample in toxtyper

How to use ToxTyper

1. Launch ToxTyper by clicking on toxtyper icon. 

2. Answer "Yes" to "Start HyStar Automatically"

3. System access at Compass OpenAccess

4. Click on Queue Job(s)

5. Log in with user name and password then click "Next>". Now use demo.

6. Supply sample name (ID) and select Method for analysis (doa = drug of abuse, toxtyper = general, toxtyper QC = QC standard for instrument check)

7. Place sample vial on the specified position (R = Red, G = Green, B = Blue tray) , mark position and click Finish

8. The run will start 

Solvent Preparation for ToxTyper

Preparation of Mobile phase:

Mobile Phase A (for 100mL):

1. Water 97 mL
2. ACN 1 mL
3. 100 mM ammonium formate 2 mL
4. Conc Formic acid 0.1 mL

Mobile Phase B (for 100mL):

1. ACN 98 mL
2. 100 mM ammonium formate 2 mL
3. Conc Formic acid 0.1 mL

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Preparing 100mL of 100 mM ammomium formate

1. Add 50 mL water to volumetric flask
2. Add 381 uL of conc formic acid
3. Add 747 uL of 25% ammonia
4. Add water to make 100 mL

*** Rear Seal Wash solvent is 10 % MeOH

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Calculation for preparing ammonium formate from ammonia and formic acid

1. formic acid density = 1.22 g/l

2. concentration in % = 98-100%

3. Molecular weigth = 46

Molar conc = 10*d*x/M = 10*1.22*99%/46 = 26.25 M

1. ammonia density = 0.91 g/l

2. concentration in % = 25%

3. Molecular weigth = 17

Molar conc = 10*d*x/M = 10*0.91*25%/17 = 13.38 M

For 100mL 100mM Ammonium formate (ratio = 1:1), use formic acid = 0.01 mol => 0.01 mol/26.25M = 0.381 mL; use ammonia = 0.01 mol => 0.01 mol/13.38M = 0.747 mL