start easy-nanosprayer on maXis

1. Replace the normal spacer with "Low Flow spacer".
2. Check the tip of the sprayer if the core capillary is fully retracted in the steel tube.
3. Insert the nanospray assembly on to the ESI source.
4. Turn the gold wheel clockwise to extend the core capillary (can't observe).
5. Connect syringe pump with tune mix (L ESI-TOF version) flow at 60 uL/h)
6. Adjust nebulizer as required.

The easy-nanospray needs to be primed with 1uL/min flow of mobile phase or tune mix solution. I prefer the latter, as I can check and recalibrate the system at the same time. At 1 uL/min flow, nebulizer was set as low as 0.1 bar to achieve the most intense signal.