internal standard for peptide digest

Add 10 uL of glu-fib 500 fmol/uL for 100 uL of sample (at 100fmol/uL). Works fine.

start easy-nanosprayer on maXis

1. Replace the normal spacer with "Low Flow spacer".
2. Check the tip of the sprayer if the core capillary is fully retracted in the steel tube.
3. Insert the nanospray assembly on to the ESI source.
4. Turn the gold wheel clockwise to extend the core capillary (can't observe).
5. Connect syringe pump with tune mix (L ESI-TOF version) flow at 60 uL/h)
6. Adjust nebulizer as required.

The easy-nanospray needs to be primed with 1uL/min flow of mobile phase or tune mix solution. I prefer the latter, as I can check and recalibrate the system at the same time. At 1 uL/min flow, nebulizer was set as low as 0.1 bar to achieve the most intense signal.

Integration of Shimadzu Prominence HPLC to HyStar

1. Back up system
2. Connect Network Card #1 to CBM-20A Lite (with or without Ethernet Switch)
3. Setup a static IP address 10.104.52.20 for Network card #1
4. Install the Shimadzu-HyStar plug-in
5. In HyStar's hardware setup, choose Shimadzu HPLC as the LC System
6. Click LC System Settings
7. Choose CBM-20A Lite as controller, click "configure"
7. Specify IP address of 10.104.52.14 for CBM-20A Lite Connection
8. Configure other devices (except Detector--which should be left blank)
9. Click Save
10. In HyStar hardware setup, click close.