Consumables from Bruker Daltonics On-line at http://tinyurl.com/79nmaub

I have look around for list of consumables from Bruker Daltonics. Perhaps it was my faults for not looking hard enough, but for my records, I keep it here at http://tinyurl.com/79nmaub.

In particular, below is bacteria test standards for MALDI Biotyper information and preparation protocols.
http://tinyurl.com/82bgem7


The highly sophisticated Bruker Bacterial Test Standard (BTS) contains a typical extract of Escherichia coli DH5alpha. In a MALDI-TOF mass spectrum, this extract shows a characteristic peptide and protein profile. Moreover, the extract is spiked with two additional proteins. Thus, the BTS covers an overall mass range from 3.6 to 17 kDa. Each tube contains material for the preparation of about 40 spots.


To prepare for use,
0. Prepare 50:47.5:2.5 H2O:ACN:TFA solution ("standard solvent").
1. Add 50 μl of standard solvent to the pellet and dissolve by pipetting up and down for at least 20 times at room temperature. Avoid foaming of the solution!
2. Incubate the standard for 5 min at room temperature and repeat pipetting up and down for at least 20 times.
3. Centrifuge for 2 min at room temperature (13000 rpm).
4. Prepare aliquots containing 5 μl of dissolved BTS. For this purpose, use micro tubes connected with screw caps (see above).
5. Use only one micro tube for sample preparation. Store the remaining aliquots at -18°C or below.